Anti-PDCD10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BUL8

Core Product Specifications and Parameters

Biological Background: PDCD10 Function and Localization

• PDCD10, also known as CCM3 or TFAR15, is a 25kDa protein that promotes cell proliferation and modulates apoptotic pathways. It enhances mitogen-activated protein kinase and STK26 activities, and is crucial for cell migration and normal Golgi assembly. Related references: PMID:27807006
• As a core component of STRIPAK (striatin-interacting phosphatase and kinase) complexes, PDCD10 regulates key signaling pathways, including Hippo, MAPK, nuclear receptor, and cytoskeleton remodeling, thereby influencing cell growth, differentiation, apoptosis, metabolism, and immune regulation. Related references: PMID:18782753
• PDCD10 plays an essential role in KDR/VEGFR2 signaling by increasing receptor stability and preventing its degradation, which is vital for cardiovascular development, angiogenesis, vasculogenesis, and hematopoiesis during embryogenesis.
• The protein localizes to the cytoplasm, Golgi apparatus membrane, and cell membrane, consistent with its roles in intracellular trafficking and signaling.
• It is ubiquitously expressed across tissues, reflecting its broad regulatory functions.
• Post-translational modifications of PDCD10 include acetylation and ubiquitin-like conjugation, which may modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant protein corresponding to the N-terminal 212 amino acids of human PDCD10, likely generates antibodies against multiple epitopes, which may enhance sensitivity in detecting endogenous PDCD10 across various applications.
• For Western blotting, the predicted molecular weight is 25kDa; consider using appropriate protein markers and optimize lysate preparation to avoid degradation.
• In immunohistochemistry (paraffin-embedded tissues), antigen retrieval and detection conditions should be optimized; PDCD10's ubiquitous expression may yield widespread staining.
• For immunofluorescence and immunocytochemistry, permeabilization is required due to the target's cytoplasmic and membrane localization; validate staining specificity with positive and negative controls.
• ELISA performance may depend on the coating antigen and antibody pair; consider titrating the antibody for optimal signal-to-noise ratio.
• Given the reactivity across human, mouse, and rat, verify cross-reactivity in the relevant experimental system to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for diagnostic manufacturers and researchers, offering reliable access to critical IVD raw materials like this anti-PDCD10 polyclonal antibody. For projects investigating apoptosis, angiogenesis, or STRIPAK signaling, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Streamline your development from concept to clinic with our integrated supply and technical support.