Anti-PDC Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P20941

Core Product Specifications and Parameters

Biological Background: Phosducin Function and Localization

• Phosducin (PDC), also known as 33 kDa phototransducing protein or MEKA, is a 28 kDa protein (calculated ~28.2 kDa) with multiple isoforms generated by alternative splicing.
• It participates in the regulation of visual phototransduction and the integration of photoreceptor metabolism, modulating light signal transduction in retinal photoreceptors.
• PDC inhibits the transcriptional activation activity of the cone-rod homeobox protein CRX, a master regulator of photoreceptor gene expression.
• Subcellularly, it is found in the cytoplasm, cytosol, nucleus, and enriched in photoreceptor cell compartments including the outer segment, inner segment, and connecting cilium.
• As a phosphoprotein, its activity is likely regulated by phosphorylation in response to light-dependent signaling.
• It belongs to the phosducin family and is associated with sensory transduction and vision (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen comprises the full-length sequence (aa 1-246) of human PDC, so this polyclonal antibody may detect multiple isoforms; validate the band pattern in your sample system.
• For Western blot, a predominant band near 28 kDa is expected; retinal tissue or photoreceptor-derived cell lines are recommended as positive controls.
• For IHC-P, consider antigen retrieval and test on retinal sections to confirm staining specificity in photoreceptor layers.
• The antibody cross-reacts with mouse and rat PDC, but sequence homology should be verified and performance validated in the relevant species and tissue context.
• For ELISA, optimal working dilutions for capture or detection should be determined empirically using recombinant protein or native samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials and supporting services. For phosducin-related research and immunoassay development, we supply validated polyclonal antibodies like this anti-PDC antibody, alongside antibody pairs, monoclonal alternatives, bulk ancillary reagents, and rare target sourcing. Let CamelBio streamline your workflow from discovery to clinical application.