Anti-PD-L1/CD274 Monoclonal Antibody for FC - Q9NZQ7

Core Product Specifications and Parameters

Biological Background: PD-L1/CD274 Function and Localization

• PD-L1, also known as B7-H1 or CD274, is a type I transmembrane protein belonging to the immunoglobulin superfamily.
• Functions as a ligand for the inhibitory receptor PD-1 (PDCD1), playing a critical role in maintaining immune tolerance by modulating T-cell activation thresholds and limiting effector responses (PubMed:11015443, PubMed:28813410, PubMed:28813417).
  Related references:
  PMID:11015443
  PMID:28813410
  PMID:28813417
• Through an unknown activating receptor, PD-L1 may costimulate T-cell subsets that predominantly produce IL-10 (PubMed:10581077).
  Related references:
  PMID:10581077
• In response to hypoxia, PD-L1 can translocate to the nucleus and act as a transcription coactivator, promoting GSDMC transcription and pyroptosis (PubMed:32929201).
  Related references:
  PMID:32929201
• The PD-1/PD-L1 pathway is exploited by tumors to inhibit cytotoxic T lymphocyte (CTL) function, facilitating immune evasion (PubMed:28813410, PubMed:28813417). Blockade of this pathway can reverse T-cell exhaustion, underpinning cancer immunotherapy (By similarity).
  Related references:
  PMID:28813410
  PMID:28813417
• Subcellular localization includes the cell membrane, early and recycling endosome membranes, nucleus, and secreted forms.
• Highly expressed in heart, skeletal muscle, placenta, and lung; weakly in thymus, spleen, kidney, and liver; and on activated immune cells (T-cells, B-cells, dendritic cells, monocytes).
• Keywords: Adaptive immunity, alternative splicing, glycoprotein, immunoglobulin domain, transmembrane, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 1-290) of human PD-L1, which is well-suited for generating antibodies that recognize the native conformation on live cells.
• This conjugate is optimized for direct flow cytometry; consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity in flow cytometry assays.
• For dual-color or multicolor flow cytometry, verify fluorophore compatibility and perform compensation as needed.
• Validate reactivity in your target cell line or primary cells, as PD-L1 expression can vary with activation state and microenvironment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for diagnostic raw-material sourcing, connecting manufacturers and laboratories with premium monoclonal antibodies like this anti-PD-L1 reagent. Focusing on immune checkpoint research and immuno-oncology applications, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials—simplifying your supply chain from concept to clinic.