Anti-PD-1/CD279 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q15116

Core Product Specifications and Parameters

Biological Background: PD-1/CD279 Function and Localization

• PD-1 (Programmed cell death protein 1) is an inhibitory receptor on antigen-activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Related references: PMID:21276005, PMID:31754127, PMID:32184441
• Upon ligand binding to CD274 (PD-L1) or CD273 (PD-L2), PD-1 delivers inhibitory signals that dampen T-cell activity. Related references: PMID:21276005, PMID:26602187
• Following T-cell receptor (TCR) engagement, PDCD1 associates with TCR-CD3 in the immunological synapse to directly inhibit T-cell activation. Related references: PMID:32184441
• Phosphorylation within the ITSM motif of PD-1 recruits PTPN11/SHP-2, which dephosphorylates key TCR signaling molecules such as ZAP70, PRKCQ, and CD247. Related references: PMID:32184441
• Tumors exploit the PD-1/PD-L1 pathway to attenuate anti-tumor immunity and escape destruction, with the interaction inhibiting cytotoxic T lymphocyte effector function. Related references: PMID:28951311
• Blocking the PD-1 pathway reverses the exhausted T-cell phenotype and restores anti-tumor response, providing a foundation for cancer immunotherapy. Related references: PMID:22658127, PMID:25034862, PMID:25399552
• PD-1 is localized at the cell membrane and contains immunoglobulin domains, a transmembrane helix, and undergoes post-translational modifications including phosphorylation and ubiquitination.
• Keywords from UniProt highlight its involvement in adaptive immunity, apoptosis, and its nature as a glycoprotein and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment corresponding to amino acids 205–259, located in the extracellular region of human PD-1. This design may favor recognition of native and denatured forms.
• For Western blotting, expect a band around 32 kDa under reducing conditions; however, post-translational modifications may cause slight shifts.
• In immunofluorescence (IF-P), validate fixation and permeabilization conditions in your specific cell or tissue system to preserve epitope accessibility.
• When used in ELISA, consider pairing with a matched detection antibody and validate against recombinant PD-1 standards.
• Cross-reactivity with mouse and rat is indicated; verify reactivity with your species-derived samples using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PD-1/CD279 polyclonal antibody is part of our immuno-oncology collection, enabling robust detection in assays such as WB, IF, and ELISA. Our team also specializes in the sourcing of rare-target raw materials, ensuring you have a reliable bridge from concept to clinic.