Anti-PD-1/CD279 Polyclonal Antibody for WB, IF-P, ELISA - Q02242

Core Product Specifications and Parameters

Biological Background: PD-1 Function and Localization

• PD-1 is an inhibitory receptor on activated T-cells that plays a critical role in the induction and maintenance of immune tolerance to self. Related references: PMID:10485649 PMID:11209085 PMID:11698646
• Upon binding to its ligands CD274 (PD-L1) and CD273 (PD-L2), PD-1 delivers inhibitory signals that attenuate T-cell activation. Related references: PMID:11015443 PMID:11224527 PMID:18287011
• PD-1 suppresses T-cell activation by recruiting PTPN11/SHP-2 via its phosphorylated ITSM motif, leading to dephosphorylation of key TCR signaling molecules like ZAP70, PKCθ, and CD3ζ. Related references: PMID:11698646 PMID:22641383
• PD-1 is a single-pass type I membrane protein belonging to the immunoglobulin superfamily, containing one Ig-like V-type domain.
• It is expressed in a thymus-specific manner, consistent with its role in T-cell development and tolerance.
• Tumor cells exploit the PD-1 pathway to evade immune surveillance, making PD-1 a major immune checkpoint target in cancer immunotherapy.
• PD-1 undergoes phosphorylation, ubiquitination, and glycosylation, which regulate its expression and signaling.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and validate the target band at approximately 32 kDa.
• In immunofluorescence, paraformaldehyde-fixed cell preparations are recommended; optimize permeabilization and blocking steps.
• For ELISA, pair with a suitable detection antibody and validate with known positive and negative controls.
• As a polyclonal antibody, lot-to-lot variability may occur; titration experiments are advised for each new batch.
• Ensure specificity by including appropriate isotype controls and knockout validation if possible.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immune checkpoint targets like PD-1. Our sourcing network supports rare-target procurement to accelerate your assay development from concept to clinic.