Anti-PCYT2 Polyclonal Antibody for WB, ELISA - Q99447

Core Product Specifications and Parameters

Biological Background: PCYT2 Function and Localization

• PCYT2, also known as CTP:phosphoethanolamine cytidylyltransferase or phosphorylethanolamine transferase, catalyzes the conversion of phosphoethanolamine to CDP-ethanolamine, the second step in the CDP-ethanolamine pathway for phosphatidylethanolamine (PE) synthesis.
  Related references:
  PMID:31637422
  PMID:9083101
• As a major inner-leaflet phospholipid, PE is critical for membrane structural integrity and functions, participating in cell division, cell fusion, blood coagulation, and apoptosis.
  Related references:
  PMID:31637422
  PMID:9083101
• The enzyme shows strongest expression in liver, heart, and skeletal muscle, indicating a key role in tissues with high membrane turnover or metabolic activity.
• PCYT2 is classified as a nucleotidyltransferase and is involved in lipid biosynthesis, phospholipid biosynthesis, and lipid metabolism.
• The protein undergoes phosphorylation, suggesting its activity may be regulated by post-translational modification.
• Alternative splicing generates multiple PCYT2 isoforms, potentially contributing to tissue-specific or developmental regulation.
• Mutations in PCYT2 are associated with hereditary spastic paraplegia, a neurodegenerative disorder, linking PE metabolism to neuronal health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human PCYT2 (amino acids 1–389), so the polyclonal antibody is expected to recognize native and denatured forms of the protein.
• For Western blot, a band of approximately 44 kDa is anticipated; however, alternative splicing variants may produce additional bands. Consider optimizing sample preparation and gel conditions.
• The antibody cross-reacts with mouse and rat PCYT2, enabling its use in rodent models, but epitope conservation should be verified for each species.
• In ELISA applications, this polyclonal antibody can serve as either a capture or detection reagent; however, pairing with a monoclonal antibody may enhance specificity in sandwich formats.
• Proper storage and handling during shipping (ice bag) are important to maintain antibody stability; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials, including validated antibodies like this PCYT2 polyclonal antibody. Our sourcing portfolio covers lipid metabolism targets and rare-pathway reagents, supported by technical and supply chain solutions from concept to clinic. Whether you need antibody pairs, bulk ancillary reagents, or specialized raw materials, CamelBio is your partner for efficient and reliable procurement.