Anti-PCYT1A Polyclonal Antibody for WB and ELISA - P49585

Core Product Specifications and Parameters

Biological Background: PCYT1A Function and Localization

• PCYT1A (Choline-phosphate cytidylyltransferase A, also known as CCT-alpha) is the key rate-limiting enzyme in the CDP-choline pathway for de novo phosphatidylcholine biosynthesis.
• It catalyzes the conversion of phosphocholine and CTP to CDP-choline, a critical precursor for membrane phospholipid production.
• The protein localizes to the cytoplasm, nucleus, and endoplasmic reticulum membrane, with translocation between compartments regulating its activity.
• Tissue expression is prominent in brain, placenta, liver, and both fetal and adult lung, consistent with its role in membrane synthesis.
• Post-translational modifications include acetylation and phosphorylation, and disease-associated variants are linked to cone-rod dystrophy, congenital generalized lipodystrophy, diabetes mellitus, and dwarfism (keywords from UniProt).
• Alternative splicing generates multiple isoforms, and the full-length protein has a calculated molecular weight of ~41.7 kDa, running at ~42 kDa on SDS-PAGE.
• The antibody's immunogen targets the N-terminal region (aa 1-100), allowing recognition of variants containing this epitope.

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody under reducing conditions; expect a specific band near 42 kDa in human and mouse cell lysates.
• In ELISA, titrate the antibody to determine optimal coating or detection concentrations for your assay format.
• The antibody cross-reacts with human and mouse PCYT1A; for other species, empirical verification is recommended.
• Since the immunogen is a synthetic peptide from the N-terminus, note that isoforms lacking this region may not be detected.
• Include appropriate positive and negative controls (e.g., tissue lysates known to express PCYT1A) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive IVD raw-material sourcing platform, supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to diagnostic developers worldwide. For PCYT1A-related projects in lipid metabolism and associated diseases, we offer end-to-end support from target selection to assay development. Contact us to discuss customized sourcing solutions for rare targets and streamlined procurement.