Anti-PCYT1A Monoclonal Antibody for WB, ELISA - P49585

Core Product Specifications and Parameters

Biological Background: PCYT1A Function and Localization

• PCYT1A (choline-phosphate cytidylyltransferase A, also known as CCT-alpha) is the rate-limiting enzyme in the CDP-choline pathway, catalyzing the conversion of phosphocholine and CTP to CDP-choline for de novo phosphatidylcholine biosynthesis.
• It shuttles between an inactive soluble form in the cytosol and an active membrane-bound form on the endoplasmic reticulum, with additional localization in the nucleus.
• The protein is expressed in multiple tissues, with notable presence in brain, placenta, liver, and fetal and adult lung.
• Post-translational modifications, including phosphorylation and acetylation, regulate its catalytic activity and membrane association.
• Genetic variants in PCYT1A are linked to human disorders such as cone-rod dystrophy, congenital generalized lipodystrophy, diabetes mellitus, and spondylometaphyseal dysplasia with cone-rod dystrophy (SMD-CRD).
• It belongs to the cytidylyltransferase family and contains tandem repeats essential for lipid binding and enzyme regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 268–367 of human PCYT1A, a region likely conserved and surface-exposed; consider the immunogen design for its intended applications.
• For Western blotting, use whole-cell lysates from human cell lines or tissues with high PCYT1A expression (e.g., liver, brain) and optimize detection conditions to account for the predicted ~42 kDa band.
• In ELISA, the peptide immunogen can serve as a positive control, but titration of both capture and detection reagents is recommended for optimal signal-to-noise ratio.
• Since PCYT1A localizes to multiple compartments, subcellular fractionation may be necessary if studying enzyme translocation dynamics; validate antibody performance in each fraction.

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CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a broad portfolio of IVD raw materials, including this anti-PCYT1A monoclonal antibody for phosphatidylcholine metabolism and lipid disorder studies. We streamline procurement by offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing, helping you move from concept to clinic efficiently. Reach out to explore how our supply solutions can accelerate your next project.