Anti-PCSK9 Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8NBP7

Core Product Specifications and Parameters

Biological Background: PCSK9 Function and Localization

• PCSK9 is a proprotein convertase subtilisin/kexin type 9 (also known as NARC-1, PC9) that plays a central role in cholesterol homeostasis by binding to LDL receptor family members and promoting their degradation (PubMed:18039658). Related references: PMID:18039658
• It enhances hepatic LDLR degradation via a clathrin-mediated pathway, likely by preventing recycling from endosomes or directing to lysosomes, and can induce LDLR ubiquitination (PubMed:17461796, PubMed:18197702, PubMed:18799458). Related references: PMID:17461796 PMID:18197702 PMID:18799458
• PCSK9 also inhibits APOB degradation via the autophagosome/lysosome pathway independently of LDLR, and is involved in disposal of BACE1 intermediates in the secretory pathway (PubMed:18660751). Related references: PMID:18660751
• It regulates neuronal apoptosis by modulating LRP8/APOER2 levels and associated anti-apoptotic signaling pathways.
• Subcellularly, PCSK9 localizes to the cytoplasm, secreted, endosome, lysosome, cell surface, endoplasmic reticulum, and Golgi apparatus.
• Tissue expression includes neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and Schwann cells.
• Key features include autocatalytic cleavage, alternative splicing, extensive glycosylation, phosphorylation, and sulfation; mutations are linked to familial hypercholesterolemia and hypocholesterolemia.

Experimental Guidance and Technical Tips

• This antibody is generated using a recombinant protein immunogen spanning amino acids 31-692 of human PCSK9, covering the catalytic and C-terminal domains, which may contribute to pan-PCSK9 recognition.
• For Western blotting, PCSK9 is detected as a ~74 kDa proprotein and a ~20 kDa cleaved product; consider optimizing sample preparation to visualize both forms.
• The antibody is validated for IHC-P, IF/ICC, and ELISA; validate in the relevant sample system (e.g., liver tissue for cholesterol metabolism studies) with appropriate controls.
• Cross-reactivity with mouse PCSK9 is reported; verify sequence homology for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials and supporting services. This anti-PCSK9 monoclonal antibody is part of our comprehensive portfolio for cholesterol metabolism and cardiovascular research, and we can also supply validated antibody pairs, bulk ancillary reagents, and assist with rare target sourcing. Partner with CamelBio for seamless procurement from concept to clinic.