Anti-PCSK1 Polyclonal Antibody for WB, IF/ICC, ELISA - P29120

Core Product Specifications and Parameters

Biological Background: PCSK1 Function and Localization

• Neuroendocrine convertase 1, also known as prohormone convertase 1 (PC1) or proprotein convertase 1, is encoded by the PCSK1 gene and belongs to the subtilisin/kexin-like proprotein convertase family. Alternative splicing produces multiple isoforms.
• It functions as a calcium-dependent serine endoprotease that specifically cleaves precursor proteins at sites comprised of pairs of basic amino acids (e.g., Lys-Arg, Arg-Arg), a critical step in the maturation of numerous prohormones and neuropeptides.
• Major substrates include proopiomelanocortin (POMC), proinsulin, proglucagon, prorenin, proenkephalin, prodynorphin, and proagouti-related protein (AGRP), thereby regulating energy homeostasis, stress response, and neuroendocrine signaling.
• Subcellularly, PCSK1 is localized to secretory vesicles, including dense-core granules of the regulated secretory pathway in neuroendocrine cells and neurons.
• The protein is synthesized as an inactive zymogen with an N-terminal prodomain (removed upon activation) and undergoes autocatalytic cleavage, N-glycosylation, and disulfide bond formation to generate the mature, active protease.
• Disease-associated variants in PCSK1 are linked to obesity and metabolic disorders (e.g., BMIQ12), as well as to severe malabsorptive diarrhea and endocrine abnormalities, underscoring its physiological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal portion (aa 28‑170) of human PCSK1, which includes part of the prodomain and the catalytic domain; the antibody may recognize both the full‑length precursor (~84 kDa) and processed forms.
• For Western blot, consider using positive controls from human, mouse, or rat neuroendocrine tissues (e.g., pituitary, hypothalamus, pancreatic islets) and optimize protein loading and membrane blocking conditions.
• In immunofluorescence/ICC, anticipate a punctate cytoplasmic staining pattern consistent with secretory vesicle localization; permeabilization should be sufficient to allow antibody access to intracellular compartments.
• When performing ELISA, validate the antibody pair compatibility and matrix effects in your sample type; the recombinant immunogen can serve as a positive reference standard.
• Given the cross‑reactivity with mouse and rat, this antibody is suitable for translational studies in rodent models of obesity, diabetes, or neuroendocrine dysfunction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one‑stop access to validated IVD raw materials, technical services, and consulting – from early‑stage concept through clinical application. This anti‑PCSK1 polyclonal antibody serves neuroendocrine research and biomarker development, and is part of a broad portfolio that includes optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare or emerging targets. Our team is ready to help streamline your procurement and accelerate your project timelines.