Anti-PCNX1 Polyclonal Antibody for WB, ELISA - Q96RV3

Core Product Specifications and Parameters

Biological Background: PCNX1 Function and Localization

• PCNX1 encodes pecanex-like protein 1, also designated pecanex homolog protein 1.
• The protein is predicted to be a multi-pass transmembrane protein localized to the membrane.
• It contains transmembrane helices and is classified as a glycoprotein.
• Alternative splicing generates multiple transcript variants, suggesting isoform diversity.
• As a member of the pecanex family, PCNX1 is conserved across species, though its functional role remains largely uncharacterized.
• Mass spectrometry-based proteomics has identified PCNX1 in various human tissues, indicating broad expression.
• Post-translational modifications include glycosylation, consistent with its membrane localization.

Experimental Guidance and Technical Tips

• For Western blot detection of PCNX1, the predicted molecular weight is ~259 kDa. A low-percentage polyacrylamide gel (e.g., 6–8%) and extended transfer time are recommended for efficient transfer of high-molecular-weight proteins.
• Validate antibody specificity by including appropriate positive controls (e.g., human cell lines or tissues known to express PCNX1) and negative controls.
• When performing ELISA, consider using the immunizing peptide as a positive control for coating; optimize blocking and detection conditions for this protein.
• As the antibody is a polyclonal, lot-to-lot consistency may vary; evaluate each new batch in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers with validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents. This anti-PCNX1 polyclonal antibody aids research into membrane protein biology and transmembrane glycoproteins. For projects requiring custom antibody development, rare-target sourcing, or scalable supply chains, CamelBio provides integrated technical and logistical support to bridge the gap from concept to clinical application.