Anti-PCNT Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95613

Core Product Specifications and Parameters

Biological Background: PCNT (Pericentrin) Function and Localization

• PCNT encodes Pericentrin (also known as Kendrin or Pericentrin-B), a large centrosomal protein with a molecular weight of ~378 kDa.
• Pericentrin is an integral component of the pericentriolar material (PCM) and the filamentous matrix of the centrosome, essential for organizing microtubule arrays during mitosis and meiosis.
• It cooperates with DISC1 to regulate microtubule network formation, highlighting its role in cytoskeletal dynamics.
• Pericentrin prevents premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome, maintaining genome stability.
• Subcellularly, Pericentrin localizes to the centrosome and microtubule organizing center within the cytoplasm.
• The protein is ubiquitously expressed across tissues, including placenta, liver, kidney, and thymus.
• Post-translational modifications include phosphorylation and ubiquitination; it contains coiled-coil domains and a calmodulin-binding motif, as indicated by UniProt keywords.
• Alternative splicing generates multiple isoforms, including Pericentrin-B, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment encompassing amino acids 95-212 of human PCNT, an N-terminal region likely to generate antibodies recognizing full-length Pericentrin. Consider optimizing detection conditions for the large ~378 kDa protein in Western blotting, possibly using low-percentage gels and extended transfer times.
• For immunofluorescence/immunocytochemistry (IF/ICC), expect punctate centrosomal staining; validate by co-staining with established centrosome markers such as gamma-tubulin or centrin.
• Cross-reactivity with rat PCNT ensures applicability in rodent models; verify species-specific performance in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

As your dedicated sourcing bridge, CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target antibodies like this PCNT polyclonal antibody. We support your centrosome and cell division research programs from concept to clinic, ensuring streamlined access to high-quality reagents and technical consultation.