Anti-PCNA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12004

Core Product Specifications and Parameters

Biological Background: PCNA Function and Localization

• PCNA (Proliferating cell nuclear antigen) functions as a DNA sliding clamp that enhances the processivity of DNA polymerases delta and epsilon during DNA replication (PubMed:35585232).
  Related references: PMID:35585232
• It serves as a critical scaffold in the DNA damage response (DDR), recruiting repair and tolerance factors to replication forks (PubMed:24939902).
  Related references: PMID:24939902
• Post-translational modifications modulate PCNA’s repair roles; monoubiquitination triggers translesion synthesis, while Lys-63 polyubiquitination promotes error-free template switching (PubMed:24695737).
  Related references: PMID:24695737
• Also stimulates the exonuclease and phosphodiesterase activities of APEX2 upon loading onto DNA, but not its AP endonuclease activity.
• Predominantly localizes to the nucleus, consistent with its roles in replication and repair.
• Known also as Cyclin, it is a widely used marker for cell proliferation.
• The UniProt record lists additional keywords including acetylation, phosphorylation, and ubl conjugation, highlighting extensive post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 162–261 of human PCNA, a region considered to be accessible in native conditions; this may support applications such as WB, IHC-P, IF/ICC, and ELISA as indicated.
• For western blotting, the predicted band size is approximately 29 kDa; loading controls around this weight should be selected to avoid interference.
• When using immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution should be optimized for the specific tissue type and fixation protocol.
• In immunofluorescence (IF/ICC), consider co-staining with a nuclear marker to confirm expected nuclear localization.
• Cross-reactivity with mouse and rat has been reported, but always validate in the target species and tissue to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents and rare-target sourcing. This PCNA antibody supports research in DNA replication, repair, and cell proliferation—areas central to oncology and diagnostics. Whatever your development stage, CamelBio streamlines access to the critical raw materials you need.