Anti-PCNA Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P12004

Core Product Specifications and Parameters

Biological Background: PCNA Function and Localization

• PCNA (Proliferating Cell Nuclear Antigen), also known as cyclin, is a DNA sliding clamp that confers DNA tethering and processivity to DNA polymerases and other proteins. Related references: PMID:24695737, PMID:24939902, PMID:35585232
• As an auxiliary protein for DNA polymerases delta and epsilon, PCNA increases the processivity of leading strand elongation during DNA replication. Related references: PMID:35585232
• PCNA plays a critical role in the DNA damage response (DDR) by coordinating DNA replication with repair and damage tolerance pathways. Related references: PMID:24939902
• The function of PCNA is modulated by ubiquitination: monoubiquitination recruits translesion polymerases, while Lys-63-linked polyubiquitination promotes error-free repair via recombination. Related references: PMID:24695737
• PCNA is predominantly localized in the nucleus.
• Post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination regulate PCNA's diverse functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 162-261, so the antibody may recognize a linear epitope within this region; consider this when designing antigen retrieval for IHC or denaturing conditions for WB.
• For Western Blot (WB) analysis, a band at approximately 29kDa is expected; validate the signal in your specific sample lysate.
• For immunohistochemistry (IHC-P), expect nuclear staining; optimize fixation and permeabilization conditions for your tissue type.
• For ELISA, the antibody may be used for quantitative detection of PCNA; include appropriate controls and standard curves.
• Cross-reactivity has been observed with human, mouse, rat, and African green monkey; for other species, validate experimentally.

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CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials, including monoclonal antibodies like this anti-PCNA clone. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, our platform supports your projects from concept to clinic. Let CamelBio be your procurement partner for DNA replication and repair research tools.