Anti-PCM1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15154

Core Product Specifications and Parameters

Biological Background: PCM1 Function and Localization

• PCM1 is essential for centrosome assembly and function, anchoring microtubules to the centrosome and ensuring proper localization of centrosomal proteins including CEP250, CETN3, PCNT, and NEK2. Related references: PMID:12403812 PMID:15659651 PMID:16943179

• PCM1 is critical for cilium biogenesis by recruiting the BBSome ciliary protein complex to centriolar satellites. Related references: PMID:20551181 PMID:24121310 PMID:27979967

• It also recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites, facilitating tubulin modification. Related references: PMID:34782749

• Subcellularly, PCM1 localizes to the cytoplasm, centrosome, centriolar satellites, and the cilium basal body.

• Tissue expression is detected in blood, bone marrow, breast, lymph node, ovary, and thyroid.

• The protein undergoes acetylation and phosphorylation, and is involved in chromosomal rearrangements (proto-oncogene); alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• For western blotting, PCM1 is a large (~229 kDa) protein; consider using low-percentage SDS-PAGE gels (e.g., 6% resolving) and extended transfer times with verified loading controls.
• In immunofluorescence/immunocytochemistry, expect punctate staining at centrosomes and centriolar satellites; co-staining with centrosomal markers (e.g., γ-tubulin) is recommended to confirm localization.
• For ELISA, the immunogen corresponds to the N-terminal region (aa 1-260); consider using this sequence to design peptide competition assays or as a positive control.
• Cross-reactivity has been observed with human and mouse PCM1; validate species-specific reactivity in your experimental system if using other species.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw material supplier, CamelBio offers a comprehensive portfolio of antibodies, including the anti-PCM1 polyclonal antibody, to support centrosome and cilium biology research. From validated antibody pairs to customized monoclonal and polyclonal antibody development, we provide end-to-end sourcing solutions for diagnostic manufacturers and research laboratories. Contact us to explore bulk reagents, ancillary materials, and tailored technical support for your PCM1-related projects.