Anti-PCM1 Polyclonal Antibody for WB, ELISA - Q15154

Core Product Specifications and Parameters

Biological Background: PCM1 Function and Localization

• PCM1 (Pericentriolar material 1 protein), also known as PTC4, is a large coiled-coil scaffold protein and a core component of centriolar satellites. It is required for centrosome assembly and function, ensuring the correct localization of key centrosomal proteins such as CEP250, CETN3, PCNT, and NEK2.
  Related references: PMID:12403812, PMID:15659651, PMID:16943179
• PCM1 anchors microtubules to the centrosome, maintaining the structural organization of the microtubule network.
  Related references: PMID:12403812, PMID:15659651
• PCM1 plays a critical role in cilium biogenesis by recruiting the BBSome, a ciliary protein complex, to centriolar satellites.
  Related references: PMID:20551181, PMID:24121310, PMID:27979967
• PCM1 recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites, contributing to post-translational modification of microtubules.
  Related references: PMID:34782749
• Immunofluorescence and biochemical fractionation localize PCM1 to the cytoplasm, centrosome, centriolar satellites, and cilium basal body. Tissue expression is detected in blood, bone marrow, breast, lymph node, ovary, and thyroid.
• PCM1 undergoes alternative splicing and is subject to post-translational modifications including acetylation and phosphorylation. Chromosomal rearrangements involving PCM1 are linked to proto-oncogenic fusions (e.g., RET/PCM-1), highlighting its relevance in cancer biology.

Experimental Guidance and Technical Tips

• As a polyclonal antibody raised against a synthetic peptide, pre-adsorption with the immunizing peptide may serve as a specificity control in your assay design.
• For Western blotting with cell or tissue lysates, consider starting at a standard dilution range (e.g., 1:500–1:2000) and validate in the relevant human sample system. The predicted molecular weight of PCM1 is ~229 kDa; ensure adequate gel separation and transfer conditions for high-molecular-weight proteins.
• For ELISA applications, use the immunizing peptide as a positive control and include an appropriate secondary antibody detection system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated research tools such as this anti-PCM1 polyclonal antibody. Our consolidated portfolio covers centrosome and cilium biology targets, supplemented by ancillary reagents and bulk supply options. Whether you need rare-target antibodies or complete matched-pair solutions, CamelBio bridges the gap from concept to clinic with reliable sourcing and technical support.