Anti-PCIF1 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9H4Z3

Core Product Specifications and Parameters

Biological Background: PCIF1 Function and Localization

• PCIF1 (Phosphorylated CTD-interacting factor 1), also designated CAPAM (Cap-specific adenosine methyltransferase) or hCAPAM, is encoded by the PCIF1 gene (C20orf67) and functions as an mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase. It is also known as protein phosphatase 1 regulatory subunit 121 (PPP1R121).
• Catalyzes the formation of N(6),2'-O-dimethyladenosine cap (m6A(m)) by methylating the adenosine at the second transcribed position of capped mRNAs.
  Related references:
  PMID:30467178
  PMID:30487554
  PMID:31279658
• Recruited to the early elongation complex of RNA polymerase II (RNAPII) via interaction with POLR2A, mediating co-transcriptional m6A(m) formation.
  Related references:
  PMID:30467178
• Subcellular localization is predominantly nuclear, consistent with its role in co-transcriptional mRNA processing.
• Ubiquitously expressed across tissues, reflecting its general function in mRNA cap modification.
• Post-translational modifications include phosphorylation (Phosphoprotein), and it belongs to the methyltransferase/transferase family of proteins.

Experimental Guidance and Technical Tips

• For western blotting (WB), a band of approximately 81 kDa may be detected; validate with appropriate positive and negative controls.
• In immunohistochemistry using paraffin-embedded sections (IHC-P), consider optimizing antigen retrieval conditions and antibody dilution to suit tissue and fixation methods.
• For immunoprecipitation (IP), verify that the lysis buffer and protocol preserve native PCIF1 interactions and conformation.
• As a polyclonal antibody, assess lot-to-lot consistency and consider validation of each new batch in the intended assay system.
• Cross-reactivity has been reported for human, mouse, and rat; when working with other species, confirm reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, facilitating access to validated antibodies, bulk ancillary reagents, and rare target reagents from discovery to clinical application. This anti-PCIF1 polyclonal antibody supports research into mRNA cap methylation and gene expression regulation, areas of growing diagnostic interest. Whether you require monoclonal/polyclonal antibodies, optimized antibody pairs, or custom sourcing for emerging targets, CamelBio streamlines your procurement process from concept to clinic.