Anti-PCIF1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H4Z3

Core Product Specifications and Parameters

Biological Background: PCIF1 Function and Localization

• PCIF1 (Phosphorylated CTD-interacting factor 1), also known as CAPAM (Cap-specific adenosine methyltransferase), hCAPAM, hPCIF1, C20orf67, or PPP1R121, is an mRNA (2'-O-methyladenosine-N6-)-methyltransferase.
• It catalyzes the formation of N6,2'-O-dimethyladenosine cap (m6A(m)) on the second transcribed nucleotide of capped mRNAs (PubMed:30467178, PubMed:30487554, PubMed:31279658).
  Related references: PMID:30467178, PMID:30487554, PMID:31279658
• PCIF1 is recruited to the RNA polymerase II (RNAPII) early elongation complex through interaction with POLR2A, enabling co-transcriptional m6A(m) modification.
• Subcellularly, PCIF1 localizes to the nucleus, and it is ubiquitously expressed across tissues as indicated by proteomics data.
• It belongs to the methyltransferase family and is annotated as a reference proteome component and phosphoprotein (UniProt Keywords).

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning the N-terminal region (amino acids 1-200) of human PCIF1. This region may contain functional domains relevant to its methyltransferase activity.
• For Western blot (WB), a band around 81 kDa is predicted; however, actual migration may vary. Validate specificity by using appropriate positive and negative controls (e.g., PCIF1-overexpressing or knockdown cell lysates).
• The antibody is reported to cross-react with mouse and rat PCIF1, making it suitable for studies in these model organisms. Confirm cross-reactivity in your specific samples.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution. Consider including a blocking peptide or negative control tissue to assess specificity.
• For ELISA, the recombinant immunogen can be employed as a positive control to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop IVD raw material sourcing, including validated antibodies like this anti-PCIF1 rabbit polyclonal antibody for studying mRNA cap methylation and epitranscriptomic regulation. Our product portfolio extends to optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, empowering your assay development. With end-to-end support from discovery to clinical-ready materials, CamelBio accelerates your path from concept to clinic.