Anti-PCGF6 Rabbit Polyclonal Antibody (pAb) for WB and ELISA - Q9BYE7

Core Product Specifications and Parameters

Biological Background: PCGF6 Function and Localization

• Functions as a transcriptional repressor. Related references: PMID:12167161
• May modulate histone H3K4Me3 levels by activating KDM5D histone demethylase. Related references: PMID:17320162
• Core component of the PRC1-like Polycomb group complex that monoubiquitinates histone H2A at Lys-119, facilitating chromatin compaction and stable gene silencing, including Hox gene repression during development. Related references: PMID:12167161
• Within the PRC1-like complex, regulates the E3 ubiquitin ligase activity of RNF2. Related references: PMID:26151332
• Localizes predominantly to the nucleus.
• Ubiquitously expressed across tissues.
• Contains a RING finger domain for zinc binding and ubiquitin ligase activity, along with a coiled-coil region; undergoes phosphorylation and isopeptide bond formation.
• The gene undergoes alternative splicing, producing multiple transcript variants.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band around 39 kDa; nuclear extracts are recommended given the nuclear localization.
• For ELISA, consider using recombinant PCGF6 protein as a standard.
• Cross-reactivity with mouse and rat has been observed; validate in the relevant sample system.
• Optimize antibody dilution for each application using known positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects involving epigenetic regulation and chromatin biology, we can source rare-target reagents like this anti-PCGF6 polyclonal antibody, ensuring reliable supply from concept to clinic. Our technical team assists with custom sourcing and consultation to accelerate your assay development.