Anti-PCGF1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BSM1

Core Product Specifications and Parameters

Biological Background: PCGF1 Function and Localization

• PCGF1 (Polycomb group RING finger protein 1), also known as NSPc1 (Nervous system Polycomb-1) and RNF68, is a core component of the Polycomb group (PcG) multiprotein BCOR and PRC1-like complexes, which maintain transcriptional repression of key genes such as BCL6 and CDKN1A.
• It functions as a transcriptional repressor by binding to the CDKN1A promoter via the retinoic acid response element (RARE), thereby downregulating CDKN1A expression to promote cell cycle progression and proliferation, and potentially contributing to tumor cell growth.
• Within the PRC1-like complex, PCGF1 is essential for chromatin remodeling through mediating monoubiquitination of histone H2A at Lys-119, rendering chromatin heritably repressed.
  Related references: PMID:26151332
• PCGF1 regulates RNF2 ubiquitin ligase activity within the PRC1-like complex, further fine-tuning histone H2A ubiquitination.
  Related references: PMID:26151332
• It also regulates the expression of pluripotency factors DPPA4 and NANOG in embryonic carcinoma cells, indicating a role in maintaining stemness.
  Related references: PMID:26687479
• Subcellularly, PCGF1 localizes to the nucleus, consistent with its role in transcriptional regulation and chromatin modification.
• PCGF1 is ubiquitously expressed across tissues, reflecting its fundamental epigenetic regulatory functions.
• The protein undergoes various post-translational modifications including acetylation and phosphorylation, and contains a zinc-finger domain necessary for its function, as highlighted by keywords such as Metal-binding and Zinc.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 160-259 of human PCGF1, a region that may be conserved across species; the antibody's cross-reactivity with human, mouse, and rat suggests successful detection in these models.
• For Western blot (WB), a band at approximately 30 kDa is expected based on the predicted molecular weight; consider optimizing lysate preparation and blocking conditions to reduce background.
• In immunofluorescence/immunocytochemistry (IF/ICC), nuclear localization of PCGF1 should be observed; ensure proper fixation and permeabilization for nuclear antigen accessibility.
• For ELISA, validate the antibody against the specific immunogen or recombinant protein to establish working concentrations.
• Since PCGF1 is part of multi-subunit complexes, co-immunoprecipitation may be challenging due to potential interference with complex integrity; consider testing in denaturing or non-denaturing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, connecting diagnostic manufacturers and research laboratories with high-quality antibodies, including this PCGF1 rabbit polyclonal antibody optimized for applications such as WB, IF/ICC, and ELISA. We specialize in sourcing validated antibody pairs, bulk ancillary reagents, and rare-target raw materials, streamlining your journey from research concept to clinical application. Whether you need this PCGF1 antibody or additional reagents for epigenetics, cancer biology, or developmental studies, CamelBio provides a seamless procurement experience.