Anti-PCF11 Rabbit Polyclonal Antibody for WB, ELISA - O94913

Core Product Specifications and Parameters

Biological Background: PCF11 Function and Localization

• PCF11 (Pre-mRNA cleavage complex 2 protein Pcf11) is the human homolog of yeast Pcf11, a key component of the pre-mRNA cleavage complex II.
• It plays a critical role in 3' end processing of pre-mRNA and promotes transcription termination by RNA polymerase II.
• PCF11 localizes exclusively to the nucleus, consistent with its function in mRNA maturation.
• The protein is subject to multiple post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination (isopeptide bond), as annotated in UniProt.
• Structural features include a coiled-coil domain; partial 3D structures have been resolved.
• PCF11 has a calculated molecular weight of 173 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, the expected band size is approximately 173 kDa. Consider using nuclear extracts from human or mouse cells as a positive control.
• The immunogen (aa 1256-1555) targets the C-terminal region; verify reactivity in relevant sample types and applications.
• As a polyclonal antibody, it may recognize multiple epitopes; validate specificity in your experimental system.
• For ELISA, optimize coating antigen and detection conditions based on the immunogen sequence.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials for mRNA processing and transcription research. We offer validated antibody pairs, bulk polyclonal antibodies, and ancillary reagents to support your assay development. Our supply network covers rare-target sourcing, helping you advance from concept to clinical application seamlessly.