Anti-PCDHB4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9Y5E5

Core Product Specifications and Parameters

Biological Background: PCDHB4 Function and Localization

• PCDHB4 (Protocadherin beta-4) is a member of the protocadherin beta gene cluster, which encodes cadherin-related cell adhesion proteins.
• It functions as a potential calcium-dependent cell-adhesion protein, mediating homophilic cell-cell interactions.
• May play a role in the establishment and maintenance of specific neuronal connections in the brain.
• Localizes to the cell membrane, with a single-pass transmembrane domain and an extracellular region containing cadherin repeats.
• Predicted to be a glycoprotein with a signal peptide and multiple calcium-binding sites.
• Identified through proteomics approaches and classified as a reference proteome member.

Experimental Guidance and Technical Tips

• Based on the immunogen derived from the extracellular domain (aa 28-300), this antibody may detect the extracellular region of PCDHB4 in native conformations for IF/ICC.
• For Western blot, a band corresponding to the ~87 kDa full-length protein is expected; additional bands may appear due to glycosylation or processing.
• Validate antibody performance in relevant neuronal cell lines or brain tissue lysates, as PCDHB4 is predominantly expressed in the brain.
• Include appropriate controls (e.g., knockdown lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For neuroscientists studying cell adhesion and neuronal connectivity, we offer validated antibody pairs, optimized polyclonal antibodies like this anti-PCDHB4 rabbit pAb, and bulk ancillary reagents. Our sourcing network ensures even rare targets like protocadherin beta-4 are accessible for your assay development needs.