Anti-PCDHB16 Polyclonal Antibody for WB, ELISA - Q9NRJ7

Core Product Specifications and Parameters

Biological Background: PCDHB16 Function and Localization

• PCDHB16 (Protocadherin beta-16, also known as PCDH3X) is encoded by the PCDHB16 gene (KIAA1621) in the protocadherin beta cluster on human chromosome 5.
• It is a potential calcium-dependent cell-adhesion protein, possibly contributing to the establishment and maintenance of specific neuronal connections in the brain.
• Subcellular localization is predicted to be at the membrane, consistent with its role in cell-cell interaction.
• Structurally, PCDHB16 contains extracellular cadherin-like repeats, a single transmembrane helix, and a cytoplasmic domain, as inferred from conserved domain architecture and UniProt keywords (Repeat, Transmembrane, Transmembrane helix).
• The protein is classified as a glycoprotein and carries a signal peptide, indicating processing through the secretory pathway.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 29–90 of human PCDHB16 (extracellular region), supporting antibody specificity for the ectodomain.
• For WB, the expected molecular weight is approximately 85 kDa; consider loading appropriate positive controls (e.g., brain tissue lysates from human, mouse, or rat) and validating band specificity with competitive blocking.
• In ELISA applications, use the recombinant immunogen as a positive control and include negative controls to assess background. Optimize coating concentrations and detection antibodies empirically.

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CamelBio streamlines IVD raw-material procurement by offering validated antibody pairs, optimized polyclonal antibodies like this anti-PCDHB16 pAb, and bulk ancillary reagents. For neuroscience and cell adhesion research, we can also source rare target proteins and custom antibody development services. Let us be your partner from concept to clinic.