Anti-PCDHA13 Polyclonal Antibody for WB, ELISA - Q9Y5I0

Core Product Specifications and Parameters

Biological Background: Protocadherin alpha-13 Function and Localization

• Protocadherin alpha-13 (also known as CNRS5) is a member of the cadherin superfamily, functioning as a potential calcium-dependent cell-adhesion protein.
• It may be involved in the establishment and maintenance of specific neuronal connections in the brain, contributing to synaptic specificity and neural circuit formation.
• Encoded by the PCDHA gene cluster, the gene undergoes complex alternative splicing to generate diverse protocadherin isoforms with distinct extracellular domains but a common cytoplasmic tail.
• The protein is a single-pass type I membrane protein localized to the cell membrane, consistent with its role in cell-cell adhesion.
• It contains six cadherin repeats, a transmembrane helix, and a short cytoplasmic domain, and is classified as a glycoprotein, likely undergoing post-translational modifications.
• Associated keywords include calcium-dependent adhesion, alternative splicing, and neuronal connectivity, highlighting its specialized function in the nervous system.

Experimental Guidance and Technical Tips

• For Western blot, expect a band near the predicted molecular weight of ~102 kDa. Use appropriate positive control lysates from human, mouse, or rat brain tissues or neuronal cell lines.
• In ELISA applications, consider coating recombinant PCDHA13 protein (e.g., the immunogen fragment) as a standard. Titrate the antibody carefully to optimize signal-to-noise ratio.
• As a polyclonal antibody raised against a partial recombinant protein, it may detect multiple protocadherin alpha isoforms; verify specificity to PCDHA13 if critical for your experiment.
• The antibody is shipped on ice bags; store at -20°C upon receipt and follow standard sample preparation protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive supply channel for IVD raw materials. This PCDHA13 polyclonal antibody supports neuroscience research into neuronal adhesion and connectivity, and our sourcing capabilities extend to validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw materials to streamline your assay development.