Anti-PCDHA12 Rabbit Polyclonal Antibody for WB and ELISA - Q9UN75

Core Product Specifications and Parameters

Biological Background: Protocadherin Alpha-12 Function and Localization

• Protocadherin alpha-12 (PCDHA12) is a member of the protocadherin alpha gene cluster, which encodes diverse cell adhesion molecules predominantly expressed in the nervous system.
• PCDHA12 is a potential calcium-dependent cell-adhesion protein, likely mediating specific intercellular connections through homophilic interactions.
• It may play a role in the establishment and maintenance of specific neuronal connections in the brain, contributing to synaptic specificity and neural circuit formation.
• The protein is a single-pass type I transmembrane glycoprotein, anchored at the cell membrane with an extracellular cadherin domain and a cytoplasmic tail.
• PCDHA12 undergoes alternative splicing, generating multiple isoforms that may diversify its adhesive properties and functional roles.
• Key structural features include calcium-binding repeats, a signal peptide, and a transmembrane helix, consistent with its role as a classical cadherin-related adhesion molecule.

Experimental Guidance and Technical Tips

• For Western blot, consider using a low-percentage gel (e.g., 8% SDS-PAGE) and optimize transfer conditions for high molecular weight proteins (~102kDa).
• Validate antibody specificity in neuronal tissue lysates or cell lines known to express PCDHA12; be aware of potential cross-reactivity with other protocadherin alpha family members due to high sequence homology.
• For ELISA, use recombinant PCDHA12 protein as a positive control and titrate the antibody to achieve optimal signal-to-noise ratio.
• Since the immunogen corresponds to a region near the C-terminus (amino acids 713-792), it may recognize multiple isoforms if the epitope is conserved; verify with appropriate controls.

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CamelBio provides diagnostic manufacturers and research labs with one-stop access to high-quality IVD raw materials, including validated antibodies like this anti-PCDHA12 polyclonal antibody for neuroscience and cell adhesion research. Our supply portfolio covers optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing, supporting your workflow from concept to clinic. Let us streamline your procurement and accelerate your development.