Anti-PCDH9 Polyclonal Antibody for WB, ELISA - Q9HC56

Core Product Specifications and Parameters

Biological Background: Protocadherin-9 Function and Localization

• Protocadherin-9 (PCDH9) is a member of the cadherin superfamily, which are calcium-dependent cell-cell adhesion proteins.
• The protein is predicted to contain multiple extracellular cadherin repeat domains, a transmembrane segment, and a cytoplasmic tail.
• PCDH9 is a potential calcium-dependent cell-adhesion protein, mediating cell-cell interactions in a Ca²⁺-dependent manner.
• It is localized to the cell membrane, functioning as a single-pass type I membrane protein.
• The UniProt keywords include "Alternative splicing", indicating the existence of multiple transcript variants encoding different isoforms.
• Post-translational modifications include glycosylation, as indicated by the "Glycoprotein" keyword.
• The protein contains a signal peptide for targeting to the secretory pathway, and the mature protein is anchored to the membrane via the transmembrane helix.
• PCDH9 has a predicted molecular weight of approximately 136 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 400-600 of human PCDH9 (NP_982354.1). Verify the epitope conservation across isoforms if multiple variants are relevant to your experiment.
• For Western blot, the predicted molecular weight is 136 kDa; consider using an appropriate gel concentration (e.g., 6-8% polyacrylamide) for efficient separation of high-molecular-weight proteins and optimize transfer conditions accordingly.
• Validate antibody specificity in your sample type by including appropriate positive and negative controls, as cross-reactivity with other protocadherin family members is possible.
• For ELISA applications, consider direct antigen coating or sandwich ELISA formats; titrate the antibody to determine optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material sourcing partner, CamelBio provides diagnostic manufacturers and laboratories with high-quality antibodies and ancillary reagents. This anti-PCDH9 polyclonal antibody supports research in cell adhesion and calcium signaling, areas critical for understanding developmental biology and disease. Beyond this product, CamelBio offers validated antibody pairs, bulk monoclonal and polyclonal antibodies, and customized sourcing for rare targets, simplifying your path from concept to clinic.