Anti-PCDH15 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q96QU1

Core Product Specifications and Parameters

Biological Background: Protocadherin-15 Function and Localization

• Protocadherin-15 is a member of the cadherin superfamily, functioning as a calcium-dependent cell adhesion protein essential for normal retinal and cochlear function.
• Mutations in PCDH15 are associated with Usher syndrome type 1F (USH1F), a form of deaf-blindness, and non-syndromic deafness DFNB23, highlighting its critical role in hearing and vision.
• The protein exists in multiple isoforms generated by alternative splicing, including CD1, CD2, and CD3 subtypes, which exhibit distinct tissue expression patterns.
• Subcellular localization includes the cell membrane and secreted compartments; in retina and cochlea, it is found in synaptic layers, nerve fiber layers, and supporting cells.
• Tissue expression is broad, including brain, lung, kidney, spleen, and testis, with notable expression in cytotoxic tumor-derived T- and NK-cell lines but not in normal peripheral blood cells.
• The protein features extracellular cadherin repeats, a transmembrane helix, a signal peptide, and undergoes glycosylation and disulfide bond formation.
• Clinically relevant keywords include deafness, retinitis pigmentosa, non-syndromic deafness, and Usher syndrome, underscoring its importance in sensory disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 160–400 of human PCDH15, a region likely within the extracellular domain, which may be conserved across species given cross-reactivity with mouse and rat.
• For Western blot (WB), consider using reduced whole-cell lysates or tissue homogenates; a band around 216 kDa should be anticipated, but isoform-specific or cleavage products may appear.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; positive staining is expected in retina, cochlea, and testis.
• This polyclonal antibody may be suitable for ELISA-based quantification, though pairing with a capture antibody should be validated.
• Cross-reactivity with mouse and rat enables application in rodent models of Usher syndrome or hearing loss; confirm specificity in the relevant tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop partner for diagnostic manufacturers and research institutions, providing high-quality IVD raw materials and technical support from concept to clinic. This anti-PCDH15 polyclonal antibody is ready for integration into assays studying hearing loss, vision disorders, and related sensory pathologies. Beyond individual antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assist in sourcing rare target raw materials to accelerate your diagnostic development.