Anti-PCDH1 Polyclonal Antibody for WB and ELISA - Q08174

Core Product Specifications and Parameters

Biological Background: PCDH1 Function and Localization

• Protocadherin-1 (PCDH1), also known as cadherin-like protein 1 and PC42, is a member of the cadherin superfamily, characterized by extracellular cadherin repeats.
• It may play a role in cell-cell adhesion and interaction processes, contributing to tissue organization and signaling.
• PCDH1 belongs to the non-clustered protocadherins, a subgroup distinct from the clustered protocadherins, with functions in cell adhesion and signal transduction.
• The protein is localized to cell junctions and the cell membrane, as indicated by subcellular localization data.
• Key structural features include a signal peptide, extracellular cadherin repeats, a transmembrane helix, and calcium-binding domains, essential for its adhesive function.
• Post-translational modifications: It is a glycoprotein and phosphoprotein, with alternative splicing producing multiple isoforms.
• Tissue specificity: PCDH1 is highly expressed in the brain and neuro-glial cells, suggesting a role in neural development and function.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain (aa 60–220) of human PCDH1, making it suitable for detecting both full-length and potential soluble isoforms in WB and ELISA.
• For Western blot, a band corresponding to the predicted molecular weight of ~115 kDa may be observed; validate specificity using relevant positive and negative cell lines or tissues.
• Optimize antibody dilution empirically for WB (e.g., 1:500–1:2000) and ELISA (e.g., 1:1000–1:5000) and include appropriate controls.
• Consider using blocking solutions containing 5% non-fat milk or BSA to reduce non-specific binding.
• For ELISA, coat the antigen in carbonate/bicarbonate buffer and follow standard titration protocols to determine working antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, offering streamlined procurement of IVD raw materials. This rabbit polyclonal anti-PCDH1 antibody supports investigations into cell adhesion and neurobiology, complementing our range of validated antibodies for studies of cadherin-mediated processes. Beyond individual reagents, CamelBio can supply optimized antibody pairs, bulk ancillary reagents, and customized sourcing for difficult or rare targets, ensuring reliable supply from concept to clinical application.