Anti-PCCA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P05165

Core Product Specifications and Parameters

Biological Background: Propionyl-CoA Carboxylase Alpha Chain Function and Localization

• The PCCA gene encodes the alpha subunit of propionyl-CoA carboxylase, a mitochondrial biotin-dependent enzyme. Alternative splicing produces multiple isoforms.
• Catalyzes the ATP-dependent carboxylation of propionyl-CoA to D-methylmalonyl-CoA, which is essential for the degradation of odd-chain fatty acids and branched-chain amino acids (isoleucine, threonine, methionine, valine) (PubMed:10101253, PubMed:6765947, PubMed:8434582). Related references: PMID:10101253, PMID:6765947, PMID:8434582
• Also acts on butyryl-CoA to generate ethylmalonyl-CoA, albeit at a reduced rate (PubMed:6765947). Related references: PMID:6765947
• Minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA (By similarity).
• Localized to the mitochondrial matrix, where it participates in the catabolic pathways mentioned.
• Post-translational modifications include biotinylation at the active site, acetylation, and phosphorylation, which may regulate enzyme activity.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 449–728) of human PCCA; antibodies raised against this region may recognize multiple isoforms.
• For WB, a band at approximately 80 kDa is expected; consider using a molecular weight marker and a loading control.
• Validate species cross-reactivity in the intended sample matrix, as reactivity with mouse and rat is reported; confirmation in your specific experimental setup is advised.
• For IHC-P, titrate the antibody and perform antigen retrieval optimization; include appropriate controls such as isotype control or omission of primary antibody.
• ELISA applications may require titration to determine optimal coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material supplier, CamelBio supports diagnostic developers and research laboratories with reliable access to antibodies like this anti-PCCA polyclonal antibody for metabolic disease research and biomarker development. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing services for rare targets. Reach out to our team to align our supply capabilities with your specific R&D or manufacturing needs.