Anti-PBX3 Polyclonal Antibody for WB and ELISA - P40426

Core Product Specifications and Parameters

Biological Background: PBX3 Function and Localization

• PBX3 (Pre-B-cell leukemia transcription factor 3) is a member of the PBX homeobox protein family and acts as a transcriptional activator.
• It specifically binds the DNA sequence 5'-ATCAATCAA-3' to regulate target gene expression.
• The protein contains a conserved homeobox domain, characteristic of developmental regulators and DNA-binding transcription factors.
• Subcellular localization is predominantly nuclear, consistent with its role in transcriptional control.
• PBX3 is ubiquitously expressed across various human tissues, indicating broad regulatory functions.
• Key biological aspects include DNA binding, alternative splicing, and involvement in transcription regulation, as highlighted by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-100) of human PBX3, so the antibody is likely to recognize epitopes within this domain.
• For Western blotting, a band around 47 kDa is expected; consider using appropriate molecular weight markers and validate with positive/negative controls.
• In ELISA applications, the polyclonal nature may provide robust signal detection; optimization of antibody concentration and blocking conditions is recommended.
• Cross-reactivity with human and mouse PBX3 has been reported, but performance in other species should be experimentally verified.
• As a polyclonal antibody, lot-to-lot variability is possible; consistency should be monitored for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material sourcing by offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For your transcription and developmental biology research, we supply high-quality reagents like this PBX3 antibody, and can also assist with rare target raw-material procurement. Contact us to streamline your supply chain from concept to clinic.