Anti-PBR/TSPO Monoclonal Antibody for WB - P30536

Core Product Specifications and Parameters

Biological Background: PBR/TSPO (TSPO) Function and Localization

• PBR/TSPO, also known as Translocator protein (TSPO), peripheral-type benzodiazepine receptor (PBR), and mitochondrial benzodiazepine receptor, is encoded by the TSPO gene (GeneID: 706) and exists in multiple isoforms due to alternative splicing.
• It localizes to the mitochondrial outer membrane and is involved in the transport of porphyrins and heme, potentially binding protoporphyrin IX (by similarity).
• The protein promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism, though its precise physiological role remains controversial and it is apparently not required for steroid hormone biosynthesis. Related references: PMID:24814875
• Originally identified as the peripheral-type benzodiazepine receptor, it can also bind isoquinoline carboxamides. Related references: PMID:1847678
• TSPO is widely expressed in many tissue types, with the highest expression levels normally found in steroid-synthesizing tissues such as adrenal glands and gonads.
• Key functional keywords associated with TSPO include lipid transport, receptor activity, and mitochondrial membrane integration, highlighting its roles in cellular transport and signaling.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide corresponding to amino acids 70–169 of human TSPO. This region is highly conserved between human and mouse, which aligns with the observed cross-reactivity.
• For Western blot (WB), consider using lysates from human or mouse tissues with high TSPO expression (e.g., adrenal gland, testis, ovary). The predicted molecular weight is ~19 kDa; however, post-translational modifications may alter migration.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial localization should be confirmed with organelle-specific counterstains or markers.
• For ELISA applications, this monoclonal antibody can be utilized as a capture or detection reagent; optimal dilutions and pairing should be empirically determined.
• As a recombinant rabbit monoclonal, this antibody is expected to offer consistent lot-to-lot performance, reducing variability in immunoassays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials and technical services. For projects involving mitochondrial targets, cholesterol transport, or steroidogenic tissue biomarkers, we offer a range of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our sourcing expertise extends to rare and custom targets, ensuring your development pipeline stays on track from concept to clinic.