Anti-PBRM1 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q86U86

Core Product Specifications and Parameters

Biological Background: PBRM1 Function and Localization

• Protein polybromo-1 (PBRM1), also known as BRG1-associated factor 180 (BAF180) or polybromo-1D, is a key subunit of the PBAF form of the SWI/SNF chromatin remodeling complex.
• PBRM1 participates in transcriptional regulation by altering DNA-nucleosome topology, contributing to both gene activation and repression.
• It is essential for maintaining the stability of the SWI/SNF-B (PBAF) complex and functions as a negative regulator of cell proliferation.
• The protein contains multiple bromodomains, a DNA-binding domain, and regions for protein interactions, supporting its role in chromatin targeting.
• PBRM1 is localized to the nucleus and is widely expressed across tissues.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, which may modulate its activity and complex assembly.
• Classified as a tumor suppressor, PBRM1 is frequently mutated in various cancers, including renal cell carcinoma.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-230) of human PBRM1; it may recognize full-length PBRM1 and isoforms retaining this sequence.
• In Western blot, a major band at approximately 193 kDa is anticipated; additional bands may appear due to isoforms or degradation.
• For immunoprecipitation, use standard protocols and validate capture efficiency in the relevant cell or tissue lysate.
• ELISA performance should be confirmed with appropriate positive and negative controls; consider using recombinant protein or cell extracts.
• When working with mouse or rat samples, verify reactivity under your experimental conditions, as cross-reactivity is reported but may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PBRM1 reagent. Targeting the chromatin remodeling and cancer research fields, CamelBio can also source rare-target raw materials and provide bulk ancillary reagents to streamline your development pipeline from concept to clinic.