Anti-PBK Polyclonal Antibody for WB, ELISA - Q96KB5

Core Product Specifications and Parameters

Biological Background: PBK Function and Localization

• PBK (TOPK) is a serine/threonine-protein kinase belonging to the MAPKK family, encoded by the PBK gene, and is identified as a cancer/testis antigen (CT84).
• It phosphorylates MAP kinase p38 and is active during mitosis, suggesting a role in cell cycle regulation.
• PBK may be involved in the activation of lymphoid cells, as implicated by its identification as a lymphokine-activated killer T-cell-originated protein.
• Upon phosphorylation, PBK forms a complex with TP53 (p53), leading to TP53 destabilization and attenuation of the G2/M checkpoint during doxorubicin-induced DNA damage.
• The protein is expressed in the testis and placenta, with testicular expression restricted to the outer cell layer of seminiferous tubules.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (Ubl), which may regulate its activity and stability.
• Alternative splicing produces multiple isoforms, and the three-dimensional structure of the kinase domain has been determined, supporting functional and inhibitor studies.

Experimental Guidance and Technical Tips

• The immunogen comprises the near full-length human PBK protein (aa 1-322), which may enable detection of multiple isoforms.
• For Western blot, validate the antibody in positive control lysates such as testis or placenta tissues, or in synchronized cell lines to assess mitotic expression.
• In ELISA applications, consider using recombinant PBK antigen to establish assay sensitivity and specificity.
• Given the reported cross-reactivity with mouse and rat, confirm epitope conservation by aligning target sequences across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive raw-material partner for IVD reagent development, offering validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. For kinase targets like PBK, we support cancer biomarker and cell cycle research through reliable sourcing and technical consulting. Our platform streamlines procurement so you can focus on moving your diagnostic project from concept to clinic.