Anti-PBEF/Visfatin/NAMPT Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P43490

Core Product Specifications and Parameters

Biological Background: PBEF/Visfatin/NAMPT Function and Localization

• PBEF/Visfatin/NAMPT, also known as nicotinamide phosphoribosyltransferase, is a 56 kDa protein that functions as the rate-limiting enzyme in mammalian NAD biosynthesis, catalyzing the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to produce nicotinamide mononucleotide.
• In addition to its enzymatic role, the secreted form of NAMPT acts as a cytokine (PBEF) with immunomodulating properties and as an adipokine (visfatin) with potential anti-diabetic effects; this form lacks enzymatic activity due to low ATP availability in extracellular space.
• NAMPT contributes to circadian clock regulation by driving oscillatory NAD production, which modulates the activity of the CLOCK-BMAL1 heterodimer through SIRT1-mediated deacetylation, influencing rhythmic gene expression.
• The protein localizes to the nucleus, cytoplasm, and is also secreted; its subcellular distribution is consistent with its bifunctional roles in metabolism and intercellular signaling.
• NAMPT is highly expressed in bone marrow, liver, and muscle, with additional presence in heart, placenta, lung, and kidney, reflecting its broad metabolic and immunologic functions.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its enzymatic activity, stability, or secretion under different physiological conditions.
• Structurally, NAMPT belongs to the glycosyltransferase family, and its 3D structure has been resolved, providing insights into substrate binding and catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 300–400 of human NAMPT; consider epitope accessibility in native versus denatured conditions when planning experiments.
• For Western blot, a band near 56 kDa is expected; validate with appropriate lysates from human, mouse, or rat tissues, and use positive and negative controls to confirm specificity.
• For IHC-P, appropriate antigen retrieval methods may be required; consider testing on tissues with known high expression (e.g., liver, bone marrow) and include isotype controls.
• In ELISA applications, pairing this antibody with a detection reagent targeting a non-overlapping epitope could enable sandwich assay development; validate in relevant sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for IVD raw materials, supporting researchers and diagnostics developers in the field of NAD metabolism, immunology, and metabolic disorders. Our portfolio includes validated monoclonal antibodies like this anti-NAMPT clone, along with complementary antibody pairs and bulk ancillary reagents. Whether you need optimized research tools or rare target sourcing, CamelBio bridges the gap from concept to clinic with reliable supply and technical support.