Anti-PBEF/Visfatin/NAMPT Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P43490

Core Product Specifications and Parameters

Biological Background: PBEF/Visfatin/NAMPT Function and Localization

• PBEF/Visfatin/NAMPT, also known as nicotinamide phosphoribosyltransferase, pre-B-cell colony-enhancing factor 1, or visfatin, is the rate-limiting enzyme in mammalian NAD biosynthesis, catalyzing the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide.
• The protein exists in multiple forms: intracellularly as an active enzyme, and in a secreted form that acts as a cytokine (immunomodulating) and an adipokine (anti-diabetic), though the secreted form lacks enzymatic activity due to low extracellular ATP levels.
• It plays a key role in circadian rhythm regulation through NAMPT-dependent oscillatory production of NAD, which modulates CLOCK-BMAL1 transcriptional activity via NAD-dependent SIRT1-mediated suppression.
• Encoded by the NAMPT gene (also known as PBEF or PBEF1), it is a glycosyltransferase involved in pyridine nucleotide biosynthesis.
• Subcellularly, NAMPT is found in the nucleus, cytoplasm, and can be secreted into the extracellular space.
• Tissue expression is prominent in bone marrow, liver, and muscle, with additional presence in heart, placenta, lung, and kidney.
• The protein undergoes multiple post-translational modifications including acetylation and phosphorylation, which may regulate its function and localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 181-382 of human NAMPT, a region that may be accessible for antibody binding in various conformations; consider denaturing vs. native conditions based on the application.
• For WB, a band around 56 kDa is expected; validate in lysates from human, mouse, or rat samples to confirm detection.
• IHC-P and IF/ICC may require optimization of fixation and permeabilization to detect both nuclear/cytoplasmic and secreted protein pools.
• ELISA applications can be designed to capture the soluble form of NAMPT in serum or culture supernatants; include appropriate standards and controls.
• When using cross-reactive species, verify sequence homology in the immunogen region to ensure specific recognition.

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