Anti-Paxillin Rabbit Polyclonal Antibody for WB, ELISA - P49023

Core Product Specifications and Parameters

Biological Background: Paxillin Function and Localization

• Paxillin is a cytoskeletal adapter protein that localizes to focal adhesions, linking integrin signaling to the actin cytoskeleton.
• It plays a critical role in actin-membrane attachment at sites of cell adhesion to the extracellular matrix.
• Paxillin contains LIM domains and zinc-binding motifs, characteristic of its protein family.
• The protein undergoes post-translational modifications including phosphorylation and acetylation, which regulate its activity.
• It is involved in recruiting other signaling molecules such as TRIM15 to focal adhesion complexes.
• Subcellular localization includes cytoplasm, cytoskeleton, cell junction, focal adhesion, and cell cortex.
• Paxillin is subject to alternative splicing, potentially generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved N-terminal region of human Paxillin, which may account for cross-reactivity with mouse Paxillin.
• For Western blot, the expected band size is approximately 65 kDa; validate in the relevant sample system.
• For ELISA, consider testing both direct and capture formats; the polyclonal nature may provide robust signal.
• Always include appropriate controls (e.g., lysates from Paxillin-overexpressing or knockdown cells) to confirm specificity.
• Optimize antibody dilution and incubation conditions for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, from antibodies to ancillary reagents. For targets like Paxillin involved in focal adhesion and cell adhesion processes, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents to support your assay development. Our sourcing expertise extends to rare targets, ensuring you obtain the critical raw materials needed for your diagnostic pipeline.