Anti-Paxillin Monoclonal Antibody for WB, ELISA - P49023

Core Product Specifications and Parameters

Biological Background: Paxillin Function and Localization

• Paxillin is a cytoskeletal protein primarily involved in linking actin filaments to the extracellular matrix at focal adhesions.
• It serves as a scaffold to recruit signaling and structural proteins, such as TRIM15, to sites of cell adhesion, facilitating dynamic regulation of cell migration and morphology.
• Encoded by the PXN gene, paxillin undergoes alternative splicing, leading to multiple isoforms that may account for observed molecular weight variations (e.g., 65 kDa, 61 kDa, 66 kDa, 47 kDa).
• The protein localizes to the cytoplasm, cytoskeleton, and cell cortex, with enrichment at cell junctions and focal adhesions.
• Paxillin contains LIM domains and zinc-binding motifs, classifying it as a LIM domain protein involved in protein-protein interactions.
• It is subject to regulatory post‑translational modifications, including phosphorylation and acetylation, which modulate its activity and binding partners.
• As a phosphoprotein, paxillin plays a critical role in integrin signaling, linking extracellular cues to intracellular pathways controlling adhesion, spreading, and motility.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N‑terminal peptide (amino acids 1–100) of human paxillin; consider this sequence coverage when designing site‑specific detection assays.
• Recombinant rabbit monoclonal antibodies typically offer consistent specificity; nonetheless, validation in the relevant sample system (e.g., human, mouse, or rat cell lysates) is recommended.
• Western blot (WB) may detect multiple bands due to isoforms or post‑translational modifications; use appropriate controls to confirm target identity.
• For ELISA, standard titration and blocking protocols should be optimized to minimize nonspecific binding, especially in complex matrices.
• Given the cross‑reactivity across human, mouse, and rat, this antibody may be suitable for comparative studies in these species; verify reactivity with the specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers by providing reliable access to IVD raw materials like the Paxillin Rabbit mAb, a critical tool for investigating focal adhesion dynamics and cell adhesion pathways. As a comprehensive sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, andassistance in procuring rare‑target reagents to streamline your project from concept to clinic.