Anti-PAX9 Monoclonal Antibody for WB, ELISA - P55771

Core Product Specifications and Parameters

Biological Background: PAX9 Function and Localization

• PAX9 is a member of the paired box (PAX) family of transcription factors, characterized by a conserved DNA-binding paired domain.
• It functions as a transcription factor essential for normal embryonic development, particularly of the thymus, parathyroid glands, ultimobranchial bodies, teeth, skull and laryngeal skeleton, and distal limbs.
• PAX9 localizes to the nucleus, where it regulates target gene expression.
• The protein contains a paired box domain necessary for DNA binding and transcriptional regulation.
• Mutations in PAX9 have been associated with human developmental disorders.
• PAX9 is identified in proteomic studies and is a component of the reference proteome.
• The predicted molecular weight is approximately 36.3 kDa, consistent with observed band at 36 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider loading nuclear extracts to enrich PAX9 signal given its nuclear localization.
• Validate the antibody in the appropriate species and tissue context; PAX9 expression is expected in developing tooth germs, thymus, and other embryonic tissues.
• As the immunogen is a synthetic peptide near the N-terminus, the antibody likely recognizes a linear epitope; peptide competition assay can confirm specificity.
• Determine the optimal antibody dilution for your specific experimental setup; starting dilutions may require empirical testing.
• Verify cross-reactivity in mouse and rat samples if working beyond human models, as indicated by the manufacturer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutes to provide validated IVD raw materials, including this Anti-PAX9 monoclonal antibody for developmental biology and transcription factor research. We offer bulk monoclonal antibodies, optimized antibody pairs, and ancillary reagents to support your assay development and procurement needs, with the capability to source rare targets and customize solutions for your specific projects.