Anti-PAX8 Rabbit Polyclonal Antibody for WB & ELISA - Q06710

Core Product Specifications and Parameters

Biological Background: PAX8 Function and Localization

• PAX8 is a member of the paired box (PAX) family of transcription factors, characterized by a conserved paired domain involved in DNA binding.
• It functions as a key transcription factor essential for thyroid-specific gene expression, maintaining the functional differentiation of thyroid follicular cells.
• PAX8 is localized to the nucleus, consistent with its role as a transcription regulator.
• Expression is prominent in the thyroid gland, as well as in the excretory system and Wilms tumors, reflecting its developmental and disease relevance.
• Alternative splicing generates multiple isoforms, which may modulate its activity.
• The protein undergoes phosphorylation, a post-translational modification that can influence its transcriptional activity and interactions.
• PAX8 is implicated in congenital hypothyroidism when mutated, underscoring its critical role in thyroid development and function.
• It serves as a developmental protein and differentiation marker, particularly in tissues of urogenital and endocrine origin.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal peptide (amino acids 200-300) of human PAX8; consider validating antibody specificity in your target species and tissue type.
• For Western blot, PAX8 has an expected molecular weight of approximately 48 kDa; optimize sample preparation and loading controls accordingly.
• In ELISA applications, perform antibody titration and validate cross-reactivity with human, mouse, and rat samples as indicated.
• Due to PAX8's nuclear localization, include appropriate nuclear extraction protocols when analyzing protein expression.
• As multiple isoforms exist, consider using isoform-specific controls or complementary RNA-level validation to confirm detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a one-stop sourcing platform for IVD raw materials, technical services, and consulting—supporting every stage from concept to clinic. For PAX8-focused research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our robust supply chain ensures reliable procurement for studies in thyroid biology, developmental biology, and oncology.