Anti-PAX8 Rabbit Monoclonal Antibody for IF/ICC, IHC-P, ELISA - Q06710

Core Product Specifications and Parameters

Biological Background: PAX8 Function and Localization

• PAX8 is a member of the paired box (PAX) family of transcription factors, characterized by a conserved paired box DNA-binding domain.
• It functions as a master regulator of thyroid-specific gene expression, essential for maintaining the differentiated state of thyroid follicular cells.
• PAX8 is localized to the nucleus, consistent with its role in transcriptional regulation.
• It is expressed in the thyroid gland, excretory system, and Wilms tumors, highlighting its importance in both development and oncogenesis.
• Alternative splicing generates multiple isoforms, potentially modulating its transcriptional activity.
• Post-translational modifications, including phosphorylation, regulate PAX8 activity and stability.
• Mutations in PAX8 are associated with congenital hypothyroidism, underscoring its critical role in thyroid development.
• Keywords: DNA-binding, developmental protein, disease variant, phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200-300 of human PAX8; verify detection of relevant isoforms in your sample type.
• For IF/ICC and IHC-P, optimize fixation and antigen retrieval conditions, and include nuclear counterstaining to confirm expected localization.
• In ELISA, consider using the immunizing peptide as a positive control to assess antibody specificity and sensitivity.
• Cross-reactivity with mouse and rat PAX8 is indicated; validate performance in the species and tissue of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For transcription factor research targeting PAX8 and related developmental proteins, CamelBio offers validated monoclonal antibodies, optimized antibody pairs, and tailored sourcing solutions to support your assay development.