Anti-PAX7 Rabbit Polyclonal Antibody for WB, ELISA - P23759

Core Product Specifications and Parameters

Biological Background: PAX7 Function and Localization

• PAX7, also known as HuP1, is a paired box transcription factor belonging to the PAX family, characterized by a paired domain and a homeodomain that mediate DNA binding.
• It plays a critical role in muscle stem cell (satellite cell) proliferation and self-renewal, essential for myogenesis and muscle regeneration in adult tissue.
• Localized exclusively to the nucleus, PAX7 functions as a transcriptional regulator of genes involved in developmental and regenerative processes.
• The protein is subject to acetylation and other post-translational modifications that modulate its activity and stability, as indicated by proteomics identification.
• Alternative splicing generates multiple isoforms, and chromosomal rearrangements involving PAX7 are associated with certain cancers, marking it as a proto-oncogene and disease variant.
• PAX7 is also involved in developmental biology, being a key factor in neural crest and myogenic lineage specification.

Experimental Guidance and Technical Tips

• The immunogen was designed against residues 350-470 of human PAX7, a region that may be conserved across species (human, mouse, rat), supporting cross-reactivity in WB and ELISA applications.
• For Western blot, consider that PAX7 has a predicted molecular weight of ~55 kDa; validate antibody specificity in your sample system with appropriate positive and negative controls.
• In ELISA, indirect detection may be optimized by testing a range of antibody dilutions and coating buffers; recombinant PAX7 protein can serve as a standard.
• Ensure proper storage and handling: this polyclonal antibody is shipped under refrigerated conditions and should be aliquoted and stored at -20°C or below to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-PAX7 reagent. For projects in muscle biology, stem cell research, or myogenesis-related assay development, we can source bulk ancillary reagents and rare target materials from concept to clinic. Whatever your PAX7 detection needs, CamelBio streamlines your procurement with technical alignment and reliable supply.