Anti-PAX6 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P26367

Core Product Specifications and Parameters

Biological Background: PAX6 Function and Localization

• PAX6 encodes the paired box protein Pax-6, also known as aniridia type II protein or oculorhombin, a member of the paired box/homeobox transcription factor family.
• It functions as a master regulator in the development of the eye, nose, central nervous system, and pancreas, with essential roles in specifying ventral neuron subtypes and pancreatic islet alpha cell differentiation (By similarity).
• PAX6 competes with PAX4 for common promoter elements in the glucagon, insulin, and somatostatin genes, thereby modulating hormone expression (By similarity).
• It also acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity).
• The protein localizes exclusively to the nucleus.
• Alternative splicing generates multiple isoforms, and the protein is subject to ubiquitin-like conjugation (Ubl conjugation) as noted in UniProt.
• Mutations in PAX6 are associated with developmental disorders such as aniridia and Peters anomaly.
• Weak expression is detected in lymphoblasts, though the biological significance of this observation remains unclear.

Experimental Guidance and Technical Tips

• Given the immunogen spans amino acids 323–422, this antibody may recognize epitopes in the C-terminal region; users should verify reactivity in their specific experimental context.
• For Western blot, the predicted molecular weight is 46.7 kDa, but observed bands around 47–48 kDa are reported; consider running reduced and denatured samples to confirm specific signals.
• In immunohistochemistry (IHC-P), antigen retrieval conditions may need optimization for tissues known to express PAX6, such as developing eye or pancreas.
• For immunofluorescence/immunocytochemistry (IF/ICC), ensure permeabilization steps because PAX6 is a nuclear protein.
• This recombinant rabbit monoclonal antibody may require appropriate detection reagents; validate compatibility with your secondary antibody system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies like the PAX6 Rabbit mAb. Paired with our ancillary reagent supply and expert technical consulting, we accelerate the transition of novel targets into reliable diagnostic assays. For tailored sourcing of rare-specimen or custom-validated antibodies, our dedicated bridge service ensures continuity from concept to clinic.