Anti-PAX6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P26367

Core Product Specifications and Parameters

Biological Background: PAX6 Function and Localization

• PAX6, or Paired box protein Pax-6, also known as Aniridia type II protein or Oculorhombin, is a transcription factor encoded by the PAX6 gene.
• It functions as a key developmental regulator, essential for the formation of the eye, nose, central nervous system, and pancreas.
• As a member of the paired box family, PAX6 contains both a paired domain and a homeodomain, enabling specific DNA binding to regulate target gene expression.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• PAX6 competes with PAX4 for binding to common promoter elements in the glucagon, insulin, and somatostatin genes, thereby influencing pancreatic islet cell differentiation.
• It also acts as a transcriptional repressor of NFATC1-mediated gene expression, modulating immune-related pathways.
• Alternative splicing generates multiple isoforms of PAX6, which may exhibit distinct functional properties.
• Mutations in PAX6 are associated with developmental disorders such as aniridia and Peters anomaly, highlighting its critical role in ocular development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–300 of human PAX6, a region containing the paired domain and homeodomain; this polyclonal antibody is expected to recognize multiple isoforms.
• It is suitable for Western blot (predicted band ~47 kDa, though post-translational modifications may cause shifts), immunofluorescence/immunocytochemistry (expect nuclear staining), immunoprecipitation, and ELISA.
• For optimal results, titrate the antibody concentration and validate in the relevant species (human, mouse, rat) and target tissues such as eye, brain, and pancreas.
• Consider using appropriate nuclear extraction protocols and include positive and negative controls for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a single access point for comprehensive IVD raw materials, from validated antibody pairs to optimized monoclonal/polyclonal reagents and bulk ancillary products. This PAX6 antibody supports investigations in developmental biology, neuroscience, and pancreatic research. Let CamelBio streamline your procurement process, offering expert technical support and reliable sourcing for even the most specialized targets.