Anti-PAX6 Monoclonal Antibody for WB/IHC-P/ELISA - P26367

Core Product Specifications and Parameters

Biological Background: PAX6 Function and Localization

• PAX6 encodes paired box protein Pax-6, also known as aniridia type II protein or oculorhombin, a homeobox-containing transcription factor crucial for development.
• Functions as a transcription factor essential for the morphogenesis of the eye, nose, central nervous system, and pancreas.
• Competes with PAX4 for binding to common regulatory elements in the glucagon, insulin, and somatostatin promoters, thereby influencing endocrine pancreas differentiation.
• Required for pancreatic islet alpha cell differentiation and acts as a transcriptional repressor of NFATC1-mediated gene expression.
• Localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcription factor.
• Belongs to the paired box (PAX) family and contains both a paired domain and a homeodomain, enabling sequence-specific DNA interactions.
• Expressed in lymphoblasts, though at relatively low levels, reflecting its potential role in lymphocyte biology.
• Post-translational modifications include ubiquitination, and the protein is subject to alternative splicing, generating isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 323-422) of human PAX6, suggesting reactivity with full-length protein and potentially major isoforms.
• For Western blot, expect a band at approximately 47-48 kDa; consider using nuclear extracts as PAX6 is primarily nuclear.
• In immunohistochemistry (paraffin), antigen retrieval may be necessary; validate with appropriate positive control tissues such as developing eye or brain tissues.
• For ELISA, the antibody may be used for direct detection; titre optimization and buffer conditions should be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. This anti-PAX6 antibody supports research and diagnostic development focused on transcription factors in developmental biology and oncology. We invite you to leverage our integrated supply chain to accelerate your projects from concept to clinic.