Anti-PAX5 Rabbit Monoclonal Antibody for WB, IHC-P, IP, and ELISA - Q02548

Core Product Specifications and Parameters

Biological Background: PAX5 Function and Localization

• PAX5 (Paired box protein Pax-5), also known as B-cell-specific transcription factor (BSAP), is a member of the paired box (PAX) family of transcription factors.
• It acts as a dual-function transcription factor, repressing genes inappropriate for the B-cell lineage while activating B-lineage-specific genes, a process critical for B-cell commitment. Related references: PMID:10811620 PMID:27181361
• PAX5 regulates multiple processes essential for B-cell development, including cell adhesion and migration, V(H)-to-D(H)J(H) recombination, pre-B-cell receptor signaling, and maturation to the mature B-cell stage. Related references: PMID:32612238
• It promotes global chromosomal architecture changes in pro-B cells by repressing the cohesin-release factor WAPL, thereby facilitating the generation of a diverse antibody repertoire. Related references: PMID:32612238
• During Epstein-Barr virus (EBV) infection, PAX5 maintains the viral genome copy number by promoting EBNA1/oriP-dependent binding and transcription, and it inhibits lytic EBV reactivation by modulating viral BZLF1 activity. Related references: PMID:31941781 PMID:23678172
• Subcellularly, PAX5 is localized to the nucleus, consistent with its function as a sequence-specific transcription factor.
• As indicated by UniProt keywords, PAX5 is a DNA-binding developmental protein that acts as a proto-oncogene; its alterations are frequently associated with B-cell malignancies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 200–300 of human PAX5, suggesting specific recognition of the full-length protein. For western blot (WB), standard reducing conditions are recommended; expect a band around 42 kDa. Validate in human B-cell lines (e.g., Ramos, Daudi) to confirm specificity.
• For immunohistochemistry (IHC-P), antigen retrieval (e.g., citrate buffer, pH 6.0, heat-induced) may enhance staining. Test in human lymphoid tissues where PAX5 expression is high.
• In immunoprecipitation (IP), use conditions that preserve protein interactions; consider crosslinking to stabilize transient complexes if studying weak or dynamic interactions.
• For ELISA, the peptide-based immunogen implies potential for detecting both denatured and native PAX5; empirical optimization of coating and blocking buffers is advised.
• As a rabbit monoclonal antibody, this reagent offers high lot-to-lot consistency and specificity, making it suitable for quantitative assays and long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, providing a comprehensive portfolio including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For B-cell research and diagnostic applications targeting PAX5, our team can assist with sourcing high-quality reagents tailored to your assay development needs. From concept to clinic, we support every stage of your project with reliable supply chain solutions and expert consultation.