Anti-PAX5 Polyclonal Antibody for WB, ELISA - Q02548

Core Product Specifications and Parameters

Biological Background: PAX5 Function and Localization

• PAX5 encodes the B-cell-specific transcription factor BSAP, a member of the paired box family that is essential for commitment to the B-lymphocyte lineage. Related references: PMID:10811620, PMID:27181361
• It functions as a dual regulator, repressing B-lineage inappropriate genes while simultaneously activating B-cell-specific genes, thereby coordinating early B-cell development. Related references: PMID:10811620, PMID:27181361
• PAX5 promotes V(H)-to-D(H)J(H) recombination and pre-B-cell receptor signaling, and represses the cohesin-release factor WAPL to facilitate global chromatin reorganization necessary for antibody diversity. Related references: PMID:32612238
• The protein localizes to the nucleus and undergoes alternative splicing, glycosylation, and chromosomal rearrangements that can contribute to malignancies.
• During Epstein-Barr virus (EBV) infection, PAX5 maintains viral genome copy number by promoting EBNA1/oriP-dependent activity and inhibits lytic reactivation by modulating BZLF1. Related references: PMID:31941781, PMID:23678172

Experimental Guidance and Technical Tips

• When performing Western blot, validate the antibody using nuclear extracts from B-lymphocyte cell lines (e.g., Raji, Daudi) where PAX5 is highly expressed. A band at approximately 42 kDa should be observed.
• For ELISA, consider titrating the antibody against the immunogen peptide or recombinant PAX5 protein to determine optimal coating and detection conditions.
• Given the species cross-reactivity with mouse and rat, this antibody can be employed in studies using mouse or rat models of B-cell development and lymphomagenesis.
• As a polyclonal antibody, it may recognize multiple epitopes, providing robust detection but necessitating careful control for batch-to-batch consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. As a one-stop sourcing partner, we support diagnostic manufacturers and research institutes with PAX5-focused tools for B-cell malignancy detection and immune profiling, streamlining development from concept to clinic.