Anti-PAX3 Rabbit Polyclonal Antibody for WB and ELISA - P23760

Core Product Specifications and Parameters

Biological Background: PAX3 Function and Localization

• PAX3 (Paired box protein Pax-3), also known as HuP2, is a transcription factor belonging to the paired box family and plays a critical role in early development.
• It is essential for neural development and myogenesis, regulating processes such as cell proliferation, migration, and apoptosis.
• PAX3 functions as a transcriptional activator of MITF, acting synergistically with SOX10 to control gene expression. Related references: PMID:21965087
• The protein is localized to the nucleus, consistent with its role in transcription regulation.
• PAX3 is subject to alternative splicing and phosphorylation, which may modulate its activity.
• It is associated with Waardenburg syndrome and has been linked to chromosomal rearrangements in certain cancers, highlighting its importance as a developmental protein and proto-oncogene.
• The canonical isoform has a molecular weight of approximately 53 kDa (calculated from sequence: 53 kDa; UniProt: 52,968 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 341–441) of human PAX3, which may provide specificity to the full-length protein or specific isoforms.
• For western blotting, expect a band around 53 kDa; use whole cell lysates or nuclear extracts from PAX3-expressing cell lines or tissues (e.g., developing muscle, neural crest derivatives) as positive controls.
• This antibody is suitable for ELISA; consider direct antigen coating or sandwich assay formats, optimizing the coating concentration and detection system for your target.
• Validate the antibody in your sample system, as expression levels of PAX3 can vary; induce differentiation or use known PAX3-positive models to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw material sourcing, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents for diagnostic development. Our PAX3 rabbit polyclonal antibody is ideal for transcription factor research and developmental biology applications. We supply validated antibody pairs, bulk reagents, and support for rare target sourcing to streamline your assay development from concept to clinic.