Anti-PAX1 Polyclonal Antibody for WB and ELISA - P15863

Core Product Specifications and Parameters

Biological Background: PAX1 Function and Localization

• PAX1 (Paired box protein Pax-1) is a member of the paired box (PAX) family of transcription factors, characterized by a conserved DNA-binding domain. It is also known as HuP48 and is encoded by the PAX1 gene (GeneID: 5075).
• PAX1 functions as a transcriptional activator that binds to specific DNA sequences to regulate gene expression. It is implicated in the formation of segmented embryonic structures, particularly in the development of the vertebral column (by similarity).
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• PAX1 is involved in developmental processes, and mutations in the PAX1 gene have been associated with human diseases such as congenital scoliosis, deafness, and intellectual disability (as suggested by UniProt keywords).
• Alternative splicing of the PAX1 gene produces multiple transcript variants, potentially yielding isoforms with distinct functional properties.
• Keywords associated with PAX1 include Activator, Alternative splicing, Deafness, Developmental protein, Disease variant, DNA-binding, Intellectual disability, Nucleus, Paired box, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• For Western blotting, consider using nuclear extracts from human cell lines that endogenously express PAX1. The predicted molecular weight is approximately 55 kDa, but experimental confirmation is recommended.
• For ELISA, the antibody can be used as a capture or detection reagent; validate coating conditions with the immunizing peptide or a recombinant PAX1 fragment.
• As this antibody is raised against a human-specific peptide sequence, cross-reactivity with orthologous proteins in other species is not routinely tested. Validate in the relevant sample system if non-human samples are used.
• The antibody is suitable for detection of PAX1 in human samples; endogenous expression levels may vary depending on cell type and developmental stage. Include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop sourcing solutions for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw material procurement. For developmental biology and transcription factor research, CamelBio supports your assay development from concept to clinic with reliable supply chains and technical consultation.