Anti-PASK Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96RG2

Core Product Specifications and Parameters

Biological Background: PASK Function and Localization

• PASK is a PAS domain-containing serine/threonine-protein kinase encoded by the gene PASK (KIAA0135) in humans. It is also known as STK37 or PASKIN.
• It functions as a sensor of environmental conditions such as oxygen, glucose, and nutrition, playing a role in energy homeostasis and protein translation regulation.
• PASK phosphorylates key substrates including EEF1A1 (translation elongation factor), GYS1 (glycogen synthase), PDX1 (pancreatic and duodenal homeobox 1), and RPS6 (ribosomal protein S6), thereby modulating glycogen synthesis, insulin production, and translation efficiency.
• The kinase is localized in both the cytoplasm and nucleus, consistent with its regulatory roles.
• PASK is ubiquitously expressed, with relatively higher levels in brain, prostate, and testis; at the protein level, it is present in germ cells of testis and the midpiece of sperm tails.
• It contains several conserved domains: a PAS domain (likely involved in sensing), a serine/threonine kinase domain, and a C-terminal domain that may bind lipids, as reflected by keywords such as Lipid-binding, ATP-binding, and Nucleotide-binding.
• Post-translational modifications include acetylation and phosphorylation, and the protein exists in multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 1194-1323 of human PASK (C-terminal region). This region is unique and may contribute to isoform specificity.
• This polyclonal antibody is suitable for Western blot (WB), immunohistochemistry (paraffin) (IHC-P), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA. Optimize dilutions and conditions based on your experimental system.
• For WB, a band at approximately 143 kDa is expected. Consider using a broad-range marker and appropriate gel percentage to resolve this high molecular weight protein.
• Cross-reactivity has been validated with human, mouse, and rat samples. For other species, check sequence homology of the immunogen region.
• The antibody is shipped on ice bags and should be stored at recommended conditions upon receipt to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials like this anti-PASK polyclonal antibody, supporting diagnostic development for metabolic, signaling, and translational research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development from concept to clinic.