Anti-PARVB Rabbit Polyclonal Antibody for WB and ELISA - Q9HBI1

Core Product Specifications and Parameters

Biological Background: PARVB Function and Localization

• PARVB encodes beta-parvin, an adapter protein also known as affixin, that belongs to the parvin family.
• It plays a key role in integrin signaling by linking integrin-linked kinase (ILK) to downstream effectors, contributing to the activation of small GTPases CDC42 and RAC1.
• PARVB is involved in actin cytoskeleton reorganization and the formation of lamellipodia, thereby regulating cell adhesion, spreading, polarity, and migration.
• Subcellular localization includes focal adhesions, cell membrane, cytoplasm, and cytoskeletal structures such as lamellipodia and sarcomeric Z-lines.
• Expression is predominantly in heart and skeletal muscle, suggesting specialized roles in muscle tissue architecture and function.
• The protein undergoes alternative splicing and phosphorylation, and contains actin-binding repeats.
• 3D structure has been determined, and it is identified in proteomics studies.

Experimental Guidance and Technical Tips

• For Western blotting, consider using positive controls from heart or skeletal muscle lysates where PARVB is highly expressed.
• Since the immunogen targets the N-terminal region (amino acids 1-100), the antibody may recognize multiple isoforms; validate in your specific sample system.
• For ELISA, the antibody can be used for capture or detection of PARVB in serum, plasma, or cell lysates; optimize coating and detection conditions.
• Non-specific binding may be reduced by titrating the antibody and using appropriate blocking buffers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, including this anti-PARVB polyclonal antibody for research in cell adhesion, migration, and integrin signaling. Beyond this product, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support rare target raw-material procurement to accelerate your diagnostic development from concept to clinic.